Blocked injection pipettes

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Blocked injection pipettes

Post  mcs on Sun Jun 15, 2008 7:18 pm

This is more a comment than a question, but we have spent the last couple of weeks struggling with blocked injection needles (DNA)and think we may have solved our problems. Out of desperation we spun our DNA at the maximum of our eppendorf (>10000g) instead of the more gentle spin recommended in the manual (2000g). After spec'ing our sample to make sure that we didn't loose our DNA we managed to inject a whole plate of oocytes without one blockage and using only a single needle. This compares to wasting 4-5 needles or more per plate with DNA spun at 2000g.

Expression levels were as high as normal and with a greater number of expressing oocytes as well.

Is there a downside that we are not aware of when spinning this hard?

Steve

mcs

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Re: Blocked injection pipettes

Post  mcs on Sun Jun 15, 2008 7:18 pm

Dear Steve

If you do not precipitate your DNA (which should not occur if you dissolve the DNA in distilled water), there should be no problem with that.

I will add this information as a possible solution for a clogged needle to the manual.

Thank you very much for the feedback, which helps us a great deal to improve our product documentation.

Christine

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