clean MEA with Caro's acid

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clean MEA with Caro's acid

Post  adamyuan on Tue Jan 10, 2012 10:03 pm

hello, everyone,

i have now ein used MEA Chip, it is covered with dead cells, i have used almost all the cleaning methods,
1) 45min, in 70 C water with washing-up cream(ph7)
2) 0.25% trypin on the MEA at 37C for 30min
3) ultrasonic baths for 15min, and 3 times in different day
but its still there, can i use Caro's acid (http://en.wikipedia.org/wiki/Peroxymonosulfuric_acid) on MEA for a short time, e.g 15min?
or maybe you have some better idea.

wating for your answer, thank you

Adam

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Re: clean MEA with Caro's acid

Post  adamyuan on Fri Jan 13, 2012 1:52 am

schon die Firma angeruft, antwordt ist nein, werde ich mit Tergazymer noch einmal versuchen.

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Re: clean MEA with Caro's acid

Post  Thomas on Sun Jan 15, 2012 7:42 pm

Hi Adam,

please consider subszantially longer digestion times for Trypsine or tergazyme. I usually leave my MEAs for 10-48hrs in the Tergazyme. (at least over night, sometimes over the weekend...)

Thomas

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Re: clean MEA with Caro's acid

Post  adamyuan on Wed Jan 25, 2012 10:13 pm

Thomas wrote:Hi Adam,

please consider subszantially longer digestion times for Trypsine or tergazyme. I usually leave my MEAs for 10-48hrs in the Tergazyme. (at least over night, sometimes over the weekend...)

Thomas

Hey Thomas,

i have tried the Tergazyme, even 3 days long, but the Chip is still covered with tissue, i think the tissue was fixed on the MEA, so how can i remove the fixed tissue?

yours

Adam

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Re: clean MEA with Caro's acid

Post  Frank MCS on Thu Jan 26, 2012 8:56 pm

Dear Adam,
if the tissue is fixed, detergent won't dissolve it, that's the idea. So either you try to remove the tissue mechanically (water jet), ot you try to find a method to neutralize the fixation. In the end, it might be easier to get a new array.

Best regards Frank
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