weird Cardiac cell signal and cells peel off the substrate

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weird Cardiac cell signal and cells peel off the substrate

Post  polaen on Tue Nov 12, 2013 10:36 pm

Hi,

I am now using MEA1060 from MCS to monitor cardiomyocyte dervived from human ES cells, sometimes I get the weird signals like in the following link,

http://i57.servimg.com/u/f57/18/61/26/26/ac112.jpg

my question is why some channels (e.g. #47/57/58) have several spikes in single beat while  other channels(e.g. #21/31/41) have single normal spike? Is it because there are multiple beating cluster above one electrode or the cells does not attach well on the electrode?

I have another question regarding the culture time, we plated the cardiomyocytes (2X10e5 cells in a well with diameter of 6mm, the MEA is treated with gelatin solution before culture) onto the MEA and get signal since day 2, however, the cell sheet may peel off soon in day 5 or day 6, can you give any suggestions?

Thanks for any help~

polaen

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Re: weird Cardiac cell signal and cells peel off the substrate

Post  Thomas on Wed Nov 13, 2013 12:54 am

Dear polaen,

thank you for sharing the results.

The problems you describe as well as the shape of the signals indicate a not optimal adhesion of the cells on the MEA surface.

Consider switching from gelatin coating to fibronectin coating.
A hydrophilic surface is critical: please use plasma treatment prior to coating 6 cell plating.

Try to have the cells just on the electrode area in a high density.

If you oike to discuss details, please feel free to give me a call under +49 7121 9092542

Thomas

Thomas

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