Use after long term storage

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Use after long term storage

Post  DiSh on Tue Mar 04, 2014 11:13 pm

Hello,

I have started to use MEA and noticed that the MEAs have been stored at 4īC in water for a long time ,
And I observe a greyish layer on the MEAs.

Can I use them as they are after ethanol cleaning or they need special cleansing.

I would be culturing hIPSc derived CMs on 6 well MEAs.

I would also like to know,
for regular continuous use, what would be the cleaning and storing procedure.

Also would you recommend  if I can autoclave the 6 well MEAs or plasma clean them.

Regards

DiSh


Last edited by DiSh on Tue Mar 04, 2014 11:14 pm; edited 1 time in total (Reason for editing : spelling error)

DiSh

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Awaiting Reply from MCS

Post  DiSh on Sun Mar 09, 2014 10:10 pm

Dear MCS,

I am eagerly awaiting a reply from the learned researchers in your team.

Regards

DiSh

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Re: Use after long term storage

Post  Thomas on Tue Mar 11, 2014 1:30 am

Dear DiSh,

- after long term storage in water MEAs should under go the regular cleaning procedure.
http://www.multichannelsystems.com/sites/multichannelsystems.com/files/documents/manuals/MEA_Manual.pdf p43ff

- MEAs can be autoclaved and plasma cleaned.

Especially after a longer storage it is recommended to perform plasma treatment and autoclaving.

Thomas

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