culturing LUHMES cells on MEA

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culturing LUHMES cells on MEA

Post  gdzenitsky on Wed Oct 29, 2014 4:15 am

Anyone have or know of a working protocol for culturing LUHMES cells on MEAs?

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Re: culturing LUHMES cells on MEA

Post  Frank MCS on Thu Oct 30, 2014 4:06 am

Hi,
sorry, I couldn't find anything in out database. I'd go for a protocol which works on cover slips and increase the initial cell density by ~50%, as attachement is usually worse on MEAs compared to glass.

Best regards Frank MCS
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Re: LUHMES culture on MEAs

Post  PdlaLu on Sat Nov 29, 2014 12:28 pm

Hi,

I have been culturing LUHMES on MEAs a few times and it always worked without problems - just coat as usual and seed a similar number to what you would normally use for a 12-well plate. Take care that medium doesn't evaporate during the following days of differentiation (placing the MEA in a dish with some water or a wet tissue paper may help). Tell me in case you need more information. Greetings!

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