Stimulation on MCS

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Stimulation on MCS

Post  DiSh1 on Mon Feb 09, 2015 3:56 am

Has any one of you tried stimulating cardiac aggregates.-

Could any one of you could tell me how you stimulate cardiac aggregates?

I wish to study ion channel behaviour by applying external stimulus on human iPSC- CM

Sincerely

DiSh1

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Cardiac Aggregates

Post  ThomasMCS on Mon Feb 09, 2015 11:17 pm

Hi DiSh,

in a first step, please determine the rate of spontaneous beating of your aggregate.
Stimulate just slightly faster in the beginning. Select an aelectrode with reasonable
good signals as a stimulation electrode. You need a electrode covered with cells and
in tight contact with the cluster.

Start with a unipolar biphasic stimulation:

Amplitude: 1V - 2V (start small, increase if no capture)
Pulse duration: 500us - 1ms per phase(start small, increase if no capture)

Add a beighbouring electrode to apply bipolar stimulation if this fails.
Apply reverse pulse tomthe neighbouring electrode.

Please use blanking circuit to minimize artifacts.
You need low impedance electrodes to deliver charge efficiently.

I hope that helped, please feel free to contact us at support@multichannelsystems.com if you need more details.

Greetings

Thomas


ThomasMCS

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