Recording from cells on coverslips flipped onto MEA

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Recording from cells on coverslips flipped onto MEA

Post  mcs on Tue Jun 10, 2008 3:01 am

Has anybody tried recording from cells cultured on a coverslip by flipping it on the MEA?

I just tried with both a 200/30 arrays, and a 3D arrays, and tried putting a relatively heavy weight on the coverslip to hold it in place.
Perhaps I still need to coat the arrays with PEI?
Or will the distance always be too great and the coupling too poor?
Has anyone else tried and had better luck, or is my attempt futile for some other reason?

If this works, it would allow for recording from cells grown in different conditions, with fewer arrays needed for culturing and waiting in the incubator!

Thank you for not laughing too much at this attempt,
Walid

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Re: Recording from cells on coverslips flipped onto MEA

Post  mcs on Tue Jun 10, 2008 3:01 am

Dear Walid

This sounds like a good idea, but I think the nutrient and oxygen supply could be a problem you should put into consideration. If cells are grown on the MEA dish, there is a high surface area. If you have the glass surface from below and from the top, there is almost no exchange.

Of course this is only from a theoretical point of view. Maybe some MEA users can add information based on their experiences as well.

Best regards, Christine (MCS)

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Re: Recording from cells on coverslips flipped onto MEA

Post  mcs on Tue Jun 10, 2008 3:01 am

<blockquote>
Thank you for your prompt response Christine,

Nutrient and oxygen supply is a good point.
I am looking for short (5 min) recordings from cells cultured on 5mm
coverslips in 96 well plates. (while waiting for the 96 well plate
recorder)
My concern has been mushing of cells and scratching of MEA surface.

To resolve the above 4 concerns, I will try culturing neurons on tissue
culture membranes, at the expense of losing visual clarity.
It is just that this method seems so obvious, that somebody must have tried it before!
I just hope they see this post and reply,
Walid
</blockquote>

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Re: Recording from cells on coverslips flipped onto MEA

Post  Branzila143 on Wed Feb 18, 2015 5:58 pm

NBs grow nicely and treatment with agents such as retinoic acid cause differentiation into a type that does demonstrate action potentials in response to electrical stimulation (via an STG2004).

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