Range of cardio myocite action potential peak voltage

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Range of cardio myocite action potential peak voltage

Post  mcs on Thu Jun 12, 2008 2:19 am

I am having to test an MEA system and I am concnered about the noise level (~ 30 microvolts peak-to-peak). Seems low but I am unsure as to what levels to expect from rat cardiomyocite cells. can anyone share a rough order of magnitude for the Action potential peak of a neonatal cardio myocite cell?

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Re: Range of cardio myocite action potential peak voltage

Post  mcs on Thu Jun 12, 2008 2:20 am

The noise level mostly depends on the electrode size and material. For a 30 m diameter electrode made fron TiN the noise level should be in the range of less than 10 V. However it is bigger for the smaller 10 m electrodes and smaller for 100M Au electrodes.

These noiselevels should not effect your recordings too much. When I record from chicken embryonic cardiomyocyte cultures, I frequently have peak to peak amplitudes of over 2mV for the depolarization component of the AP. If you are interested in the smaller repolarization component I would recommend to use the depolarisation part of the signal as a trigger and use the averager function of MC Rack. (For details how to do that please feel free to contact me)

Greetings

Thomas

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