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Culturing HL-1 cells

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Welcome to our user forum (read only mode). :: Applications Forum :: Applications and Preparations: Neuronal Electrophysiology

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Culturing HL-1 cells Empty Culturing HL-1 cells

Post  mcs Wed Jun 11, 2008 3:23 pm

Hello I have read the method about culturing and measure on cardiac HL-1 cells and I have a question: Do you also use fibronectin on the MEA-plates??

mcs

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Culturing HL-1 cells Empty Re: Culturing HL-1 cells

Post  mcs Wed Jun 11, 2008 3:24 pm

Dear eewa,

the coating on the MEAs is not to critical. As the gelatin / fibronectin coating is a bit thick (it worked however...) we use PEI coating on the MEAs (Polyethylenamine). This is very cheap and easy to do and works very nicely.

Thomas

mcs

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Culturing HL-1 cells Empty HL-1 cells

Post  jlwcolumbia Sat Mar 21, 2009 8:38 pm

Do you know where I can get this cell line?

Thanks

jlwcolumbia

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Culturing HL-1 cells Empty Re: Culturing HL-1 cells

Post  ThomasMCS Wed Apr 15, 2009 9:37 am

Hi,

cells are available from Bill Claycomb at Louisiana State University in New Orleans:
http://www.medschool.lsuhsc.edu/biochemistry/lab_claycomb.asp

Thomas

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Culturing HL-1 cells Empty Re: Culturing HL-1 cells

Post  sbahnassy17 Sun Jun 24, 2012 11:58 am

Hi there. Has anyone tried transfecting the Hl-1 cells & what protocol are you following for that?

sbahnassy17

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Culturing HL-1 cells Empty Please continue discussion in the cardiac applications forum

Post  Thomas Thu Jun 28, 2012 7:49 am

Please continue discussion in the cardiac applications forum

Thomas

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