Matrigel coating
2 posters
Matrigel coating
Hello MCS,
my lab is trying to establish a MEA recording system to study human induced pluripotent stem cell-derived neurons.
We are currently using Matrigel in our long-term cultures, which seems to work really well so far.
I would like to know if anyone has ever coated MEA chips with Matrigel? Could this interfere with the quality of the extracellular recording?
Thanks in advance!
Kind regards,
Jan
my lab is trying to establish a MEA recording system to study human induced pluripotent stem cell-derived neurons.
We are currently using Matrigel in our long-term cultures, which seems to work really well so far.
I would like to know if anyone has ever coated MEA chips with Matrigel? Could this interfere with the quality of the extracellular recording?
Thanks in advance!
Kind regards,
Jan
jr4- Posts : 3
Join date : 2012-12-09
Re: Matrigel coating
Dear Jan,
we had reports from customers that it's problematic to completely remove the matrigel from the MEAs afterwards, but nothing about impact on recording quality.
Best regards Frank MCS
we had reports from customers that it's problematic to completely remove the matrigel from the MEAs afterwards, but nothing about impact on recording quality.
Best regards Frank MCS
Frank MCS- Posts : 188
Join date : 2008-07-14
Re: Matrigel coating
Thank you Frank for the quick reply!
Do you think this issue might be resolved using a plasma cleaner?
Kind regards,
Jan
Do you think this issue might be resolved using a plasma cleaner?
Kind regards,
Jan
jr4- Posts : 3
Join date : 2012-12-09
Re: Matrigel coating
Dear Jan,
I am not sure, sorry. Maybe it's worth asking the guys who manufacture Matrigel whether plasma will dissolve it?
Best regards Frank
I am not sure, sorry. Maybe it's worth asking the guys who manufacture Matrigel whether plasma will dissolve it?
Best regards Frank
Frank MCS- Posts : 188
Join date : 2008-07-14
Re: Matrigel coating
Thank you Frank! I will let you know what the manufacturer told me.
jr4- Posts : 3
Join date : 2012-12-09
Similar topics
» MEA cleaning question
» MEA coating for islets
» Coating Protocol
» anti-bubble coating
» How to test the MEA before coating and cell culture
» MEA coating for islets
» Coating Protocol
» anti-bubble coating
» How to test the MEA before coating and cell culture
Permissions in this forum:
You cannot reply to topics in this forum