Stimulation on MCS
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Stimulation on MCS
Has any one of you tried stimulating cardiac aggregates.-
Could any one of you could tell me how you stimulate cardiac aggregates?
I wish to study ion channel behaviour by applying external stimulus on human iPSC- CM
Sincerely
Could any one of you could tell me how you stimulate cardiac aggregates?
I wish to study ion channel behaviour by applying external stimulus on human iPSC- CM
Sincerely
DiSh1- Posts : 6
Join date : 2014-07-15
Cardiac Aggregates
Hi DiSh,
in a first step, please determine the rate of spontaneous beating of your aggregate.
Stimulate just slightly faster in the beginning. Select an aelectrode with reasonable
good signals as a stimulation electrode. You need a electrode covered with cells and
in tight contact with the cluster.
Start with a unipolar biphasic stimulation:
Amplitude: 1V - 2V (start small, increase if no capture)
Pulse duration: 500us - 1ms per phase(start small, increase if no capture)
Add a beighbouring electrode to apply bipolar stimulation if this fails.
Apply reverse pulse tomthe neighbouring electrode.
Please use blanking circuit to minimize artifacts.
You need low impedance electrodes to deliver charge efficiently.
I hope that helped, please feel free to contact us at support@multichannelsystems.com if you need more details.
Greetings
Thomas
in a first step, please determine the rate of spontaneous beating of your aggregate.
Stimulate just slightly faster in the beginning. Select an aelectrode with reasonable
good signals as a stimulation electrode. You need a electrode covered with cells and
in tight contact with the cluster.
Start with a unipolar biphasic stimulation:
Amplitude: 1V - 2V (start small, increase if no capture)
Pulse duration: 500us - 1ms per phase(start small, increase if no capture)
Add a beighbouring electrode to apply bipolar stimulation if this fails.
Apply reverse pulse tomthe neighbouring electrode.
Please use blanking circuit to minimize artifacts.
You need low impedance electrodes to deliver charge efficiently.
I hope that helped, please feel free to contact us at support@multichannelsystems.com if you need more details.
Greetings
Thomas
ThomasMCS- Posts : 71
Join date : 2008-07-16
Welcome to our user forum (read only mode). :: Applications Forum :: Applications and Preparations: Cardiac Electrophysiology
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