Electroysis of electrodes

We have been recording from cardiac myocytes using standard, planar Indium Tin Oxide MEA60 plates however after only three uses we have started to see serious and rapid electrolysis of the electrodes the second the bath is grounded. The previous two attempts went flawlessly and the change has been sudden rather than gradual. All we can think of, is that the electrodes have lost their protective coating. Has any one else experienced this problem or has any ideas what may be wrong?

There are a couple of pictures available at this address showing the damage and also the electrolysis in action. Note the blue bubbles, pretty but annoying.

http://photobucket.com/albums/c334/StevenBroadbent/

Thanks

What kind of amplifier are you using? It could be that a drop of salt-containing solution short-circuits your amplifier pins (the one corresponding to the bubbling electrode). If this is the case, wipe the salty stuff gently with a tissue and distilled water.

Best wishes,

Maksym

Thanks we'll check that.

Regards.

We analyzed the problem and it seems that there is a malfunction of the amplifier, probably due to liquid spillage into the electronics, leading to voltage applied to the electrodes. We recommend a repair.
The MEA electrodes generally have a long lifetime if they are treated with care. Standard cleaning or coating procedures do not impair the electrode function.