MEA Handeling for brain slice preparations
2 posters
MEA Handeling for brain slice preparations
I have inherited an MEA-1060-BC setup. I am having trouble with noise that I believe is caused by a damaged MEA. I am considering opening a new one but before I do I would like to know if my current procedure is adequate.
What is the suggested storage and cleaning procedure for an MEA used for slice preparations? Currently after use, I rinse the MEA with ddH2O and then let dry. Once dry, I put it back in the case with the foam it was shipped with. If the MEA is visibly dirty I spray with ethanol and then rinse with ddH2O and let dry. If the contact pads need to be cleaned I spray them with ethanol and wipe with a Q-tip.
I have read that the MEA should be stored in ddH2O in the fridge when not in use. Is this necessary if I am using the MEA with hippocampal slices? Should I just do it any ways?
I have also read about hydrophillic surface coatings. Is this recomended for hippocampal slices? Currently I am using a mesh/weight to hold the slice to the electrode array.
Thank you for any help,
Everet
What is the suggested storage and cleaning procedure for an MEA used for slice preparations? Currently after use, I rinse the MEA with ddH2O and then let dry. Once dry, I put it back in the case with the foam it was shipped with. If the MEA is visibly dirty I spray with ethanol and then rinse with ddH2O and let dry. If the contact pads need to be cleaned I spray them with ethanol and wipe with a Q-tip.
I have read that the MEA should be stored in ddH2O in the fridge when not in use. Is this necessary if I am using the MEA with hippocampal slices? Should I just do it any ways?
I have also read about hydrophillic surface coatings. Is this recomended for hippocampal slices? Currently I am using a mesh/weight to hold the slice to the electrode array.
Thank you for any help,
Everet
Everet- Posts : 3
Join date : 2009-06-16
Re: MEA Handeling for brain slice preparations
As you are not culturing a hydrophilic surface is not as critical as in many other application. But it definitely helps. We use plasma cleaner treatment to hydrophilize the surface.
All Details regarding MEA Handling & Hippocampal Slice preparation you will find in the two documents linked below
http://www.multichannelsystems.com/fileadmin/user_upload/Application_Notes_Tutorials/MEA-Applications_Hippocampus.pdf
http://www.genes2cognition.org/cgi-bin/ResourceFetch?id=13
All Details regarding MEA Handling & Hippocampal Slice preparation you will find in the two documents linked below
http://www.multichannelsystems.com/fileadmin/user_upload/Application_Notes_Tutorials/MEA-Applications_Hippocampus.pdf
http://www.genes2cognition.org/cgi-bin/ResourceFetch?id=13
ThomasMCS- Posts : 71
Join date : 2008-07-16
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