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Use after long term storage
DiSh Wed Mar 05, 2014 11:13 am
Hello,
I have started to use MEA and noticed that the MEAs have been stored at 4´C in water for a long time ,
And I observe a greyish layer on the MEAs.
Can I use them as they are after ethanol cleaning or they need special cleansing.
I would be culturing hIPSc derived CMs on 6 well MEAs.
I would also like to know,
for regular continuous use, what would be the cleaning and storing procedure.
Also would you recommend if I can autoclave the 6 well MEAs or plasma clean them.
Regards
DiSh
I have started to use MEA and noticed that the MEAs have been stored at 4´C in water for a long time ,
And I observe a greyish layer on the MEAs.
Can I use them as they are after ethanol cleaning or they need special cleansing.
I would be culturing hIPSc derived CMs on 6 well MEAs.
I would also like to know,
for regular continuous use, what would be the cleaning and storing procedure.
Also would you recommend if I can autoclave the 6 well MEAs or plasma clean them.
Regards
DiSh
Last edited by DiSh on Wed Mar 05, 2014 11:14 am; edited 1 time in total (Reason for editing : spelling error)
DiSh- Posts : 3
Join date : 2014-03-04
Awaiting Reply from MCS
DiSh Mon Mar 10, 2014 10:10 am
Dear MCS,
I am eagerly awaiting a reply from the learned researchers in your team.
Regards
DiSh
I am eagerly awaiting a reply from the learned researchers in your team.
Regards
DiSh
DiSh- Posts : 3
Join date : 2014-03-04
Re: Use after long term storage
Thomas Tue Mar 11, 2014 1:30 pm
Dear DiSh,
- after long term storage in water MEAs should under go the regular cleaning procedure.
http://www.multichannelsystems.com/sites/multichannelsystems.com/files/documents/manuals/MEA_Manual.pdf p43ff
- MEAs can be autoclaved and plasma cleaned.
Especially after a longer storage it is recommended to perform plasma treatment and autoclaving.
Thomas
- after long term storage in water MEAs should under go the regular cleaning procedure.
http://www.multichannelsystems.com/sites/multichannelsystems.com/files/documents/manuals/MEA_Manual.pdf p43ff
- MEAs can be autoclaved and plasma cleaned.
Especially after a longer storage it is recommended to perform plasma treatment and autoclaving.
Thomas
Thomas- Posts : 49
Join date : 2008-06-03
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